Encounter between Present Female Characters and Neolithic Inscribed Symbols Prior to Oracle-bone Inscriptions

Inscribed symbols of Neolithic Age were sometimes suspected to be initial writing prior to developed writing system. The earliest developed writing system in China was Oracle-bone inscriptions (OBIs) of Bronze Age and researchers have long sought its predecessor. Here, we reported that two continuous symbols on a stone ax of Neolithic Liangzhu culture found their identical duplicates in a unique writing system on brocade belts woven by present women in Shanghai suburb. Women in this group duplicated the hereditary text for weddings only once each generation in the past, and they can still interpret these two characters, implying that the two identical Liangzhu symbols may have the similar meanings. The meanings and patterns are both similar to those in OBIs, suggesting that Liangzhu symbols might be one of the predecessors of OBIs. Integrating philology, genetics, linguistics, and folklore, we discussed that special small population may inherit both the genetic structure and convert culture for extremely long time, such as this population in southern Shanghai.

Transparent, Hydrolysable and Flame Retarded Bio-based Epoxy Resins via Catalyst-free Polymerization of Triglycidyl Isocyanurate and Aliphatic Diacids

In this study, transparent and hydrolysable intrinsic flame retarded epoxy resins were synthesized successfully by melting polymerization without any catalyst, simply from bio-based triglycidyl isocyanurate and aliphatic diacids. Due to the possibility of transesterification along with the ring-opening reaction, the most suitable feed ratio of [COOH]/[epoxy] is found to be 60%. By changing the carbon number of diacid from 8 to 12, ER08-60, ER10-60 and ER12-60 were synthesized. The flame retardancy of ER08-60 is found to be excellent, with a UL-94 rating at V-0 and a LOI value at 27.6%. It is revealed from this study that upon heating isocyanurate ring decomposes first and CO₂ released prevents the contact of materials with oxygen, thus preventing further combustion. The tensile strength and bending strength of ER08-60 can reach 86.6 MPa and 75.4 MPa, respectively. Additionally, all epoxy resins are able to hydrolyze quickly in both acid and alkaline solutions. It is worth to mention that these epoxy resins are transparent, with a transmittance of around 85%.

Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum

The implementation of bioprocesses in an economically feasible and industrial competitive manner requires the optimal allocation of resources for a balanced distribution between biomass formation and product synthesis. The decoupling of growth and production in two-stage bioprocesses, aiming to ensure sufficient growth before the onset of production, is particularly relevant when target products inhibit growth. In order to avoid expensive inducer molecules, continuing process monitoring, elaborate individual process optimization, and strain engineering, we developed and applied nitrogen deprivation-induced expression of genes for product biosynthesis. Two native nitrogen deprivation-inducible promoters were identified and shown to function for dynamic growth-decoupled gene expression or CRISPRi-mediated gene knockdown in C. glutamicum with superior induction factors than the standard IPTG-inducible P_trc promoter. Valorization of xylose to produce either the sugar acid xylonic acid or the sugar alcohol xylitol from xylose as sole source of carbon and energy was demonstrated. Competitive titers of up to 34 g·L⁻¹ xylonate and 13 g·L⁻¹ xylitol were achieved in two-stage processes. We discussed that the transfer to bioprocesses with C. glutamicum using carbon sources other than xylose appears straightforward in particular regarding production of growth-inhibitory compounds by their growth-decoupled fermentative production.

Fed-batch Self-regulated Fermentation of Glucose to Co-produce Glycerol and 1,3-propanediol by Recombinant Escherichia coli

As important bio-chemicals, glycerol and 1,3-propanediol (1,3-PDO) have been widely used in numerous fields, e.g., polymers, cosmetics, foods, lubricants, medicines, and so on. Bio-based 1,3-PDO is generally produced from glycerol or glucose by natural or recombinant strains, during which organic acids are always co-produced. In this work, acetic acid was also co-produced when 1,3-PDO was obtained from glucose by a recombinant strain of E. coli MG1655. Usually, a base was added to adjust the fermentation pH, resulting in the accumulation of organic salts and difficulty in the next down streaming process. Herein, a novel strategy was developed to consume the produced acetic acid by cells self in fed-batch self-regulated fermentation. The recombinant E. coli cells produced 48.33 g/L glycerol and 61.27 g/L 1,3-PDO with a total mass yield of 45.6% and without any other byproducts at the end of 5 fed-batch fermentations. The initial buffer pH, glucose concentration, pulse feeding sugar amount, time for a single batch fermentation and reducing acid were investigated by a series of comparative experiments. This fed-batch self-regulated fermentation has potential for the co-production of 1,3-PDO and glycerol, and will highlight the subsequent modification of recombinant E. coli strain by synthetic biology.

Private Property and Public Commons: Narrowing the Gap

Private property and public commons each represent strongly felt concepts of society but in very different ways. While the protection of private property is at the heart of the capitalist system and deeply embedded in our laws, the protection of the public commons is a mere subset of government policies and often lacks firm regulations. Critically, natural commons such as air, water, biodiversity, and a habitable earth, are hardly protected at all. Environmental laws regulate use and protection of natural “resources” in a strict instrumental fashion, ignoring the intrinsic value of Nature and take Earth’s ecological systems for granted. This article traces the “hidden logic” of environmental law and explores some of the history of property and the commons in the European context. It then shows the fundamental importance of ecological integrity for all efforts towards sustainable societies. The overall thesis is that property and commons must be based on ecological sustainability as a fundamental norm of law.

Comprehensive Landscape of Matrix Metalloproteinases in the Pathogenesis of Idiopathic Pulmonary Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a progressive, chronic interstitial lung disease with unknown etiology. Matrix metalloproteinases (MMPs) are involved in fibrotic lung tissues, contributing to the initiation, progression, or resolution of chronic inflammatory disease. In present study, comprehensive changes of MMPs expressions were investigated in IPF by integrative analysis of single-cell transcriptome and bulk transcriptome data. 24 of MMPs were altered and the changes could significantly distinguish IPF from normal subjects and other lung diseases. Among them, MMP1, MMP7 and MMP19 were closely associated to lung functions, susceptibility and alveolar surface density. MMP1 and MMP7 as potential diagnostic indicators, MMP1 and MMP19 as prognostic markers in IPF could accurately predict disease progression. Devolution of MMPs at single-cell resolution, MMP19 was highly expressed in macrophages and markedly interfered with TNF signaling pathway which synchronizes fibrotic microenvironment. MMP19+ macrophages were significantly different from MMP19- macrophages in energy metabolism and immune function. The interaction of MMP19+ macrophages with hyperplastic AT2 was mediated by TNFSF12-TNFRSF12A, and further activated the TNFRSF12A receptor to affect cell glucose metabolism and mitochondrial function. In summary, MMPs has great application potential in the diagnosis, treatment, and prognosis of IPF.

Roots of (and Solutions to) Our Ecological Crisis. A Humanistic Perspective

Research into the sources of contemporary ecological crisis as well as ways to overcome it has been conducted for several decades. Rich academic literature provides numerous attempts to identify the causes of the crisis and its solutions. The ecological crisis is extremely complex and variously conditioned. Therefore, I focus on determining only two sources of the crisis and, respectively, two solutions. Since the late 1960s, monotheistic religions, Christianity in particular, have been made responsible for the environmental crisis. Christianity is accused of forwarding two theses which are harmful to the environment: 1. The sole purpose of nature is to serve man. 2. By God’s will, man is endowed with unlimited power over nature. I attempt to overcome this understanding of the source of the crisis by showing the interpretation of the Bible which contradicts the above-mentioned theses. Moreover, I show “the ecological potential” of the Judeo-Christian and Muslim traditions. As the second source of the crisis I indicate modern thought: 1. Man’s alienation from nature as the result of the Cartesian division of reality into res cogitans and res extensa. 2. Francis Bacon’s program: the study of nature is the task of natural sciences alone; nature is devoid of value in itself. 3. The mathematization of nature made it possible for the natural and technical sciences to develop rapidly, which contributed to the industrial revolution. I look for an antidote to this cause of the crisis in Klaus M. Meyer-Abich’s idea of man’s peace with nature which he developed as part of the practical philosophy of nature. I believe that revealing our inseparable bond with nature and showing compassion towards nature may help overcome the destructive consequences of modern thought. 

Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of Escherichia coli

1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthetic pathway for BT from glucose via a nonessential amino acid homoserine. This non-natural pathway used an engineered phosphoserine transaminase (SerC_R42W/R77W) to achieve the deamination of homoserine to 4-hydroxy-2-oxobutanoic acid (HOBA). Three consecutive enzymes including a lactate dehydrogenase, a 4-hydroxybutyrate CoA-transferase and a bifunctional aldehyde/alcohol dehydrogenase are used to catalyze HOBA to BT. To enhance the carbon flux to homoserine, a homoserine-producing Escherichia coli was developed by improving the overexpression of two relevant key genes metL and lysC (V339A). The simultaneous overexpression of the genes encoding these enzymes for the homoserine-derived BT pathway enabled production of 19.6 mg/L BT from glucose in the homoserine-producing E. coli.

A High-efficiency Cathode Using Co₃O₄ and Carbon Paper by Electrodeposition for Rechargeable Lithium-oxygen Batteries

The conductivity, microstructure, low cost, eco-friendliness, simple and controllable preparation are key points of the preparation and application of cathode materials for lithium-oxygen batteries. Considering the above-mentioned important factors comprehensively, the Co₃O₄@CP electrode with a three-dimensional structure was prepared by directly growing Co₃O₄ on the surface of carbon paper (CP) using a simple and controllable electrodeposition method. The obtained Co₃O₄ depositing layer has a nanosheet microstructure and can provide abundant catalytic active sites for the oxygen evolution and reduction reactions. The network architecture of electronic transmission is constructed by CP in the cathode, promoting the efficiency of the electrode reaction. It’s worth noting that the binder-free and conductive additive-free cathode is beneficial to reduce side reactions. The lithium-oxygen battery assembled with the obtained Co₃O₄@CP electrode showed satisfactory electrochemical performance. The cell assembled with the obtained Co₃O₄@CP electrode provided a discharge specific capacity of 10954.7 mA·h·g⁻¹ at a current density of 200 mA·g⁻¹, and the voltage profiles of the cell were good under 100 mA·g⁻¹ at a limited capacity of 500 mA·h g⁻¹ based on the mass of Co₃O₄. Therefore, the Co₃O₄@CP composite material is a promising candidate with good application prospects as a cathode material for lithium-oxygen batteries.

Foreword to Resumption