Perspectives in Legal and Forensic Sciences Open Access

Emerging Technologies in Forensic DNA Analysis

Forensic DNA analysis has fundamentally transformed criminal investigations, providing an unprecedented level of accuracy in identifying suspects, exonerating the innocent, and solving cold cases. This manuscript reviews the emerging technologies that are reshaping the field of forensic DNA analysis, including next-generation sequencing (NGS), rapid DNA analysis, AI-driven forensic workflows, 3D genomics, and mobile DNA platforms. These innovations enhance the speed, precision, and scope of DNA analysis, allowing forensic scientists to process evidence more efficiently, analyze more complex samples, and conduct real-time field-based investigations. While these advancements hold great promise, they also introduce significant challenges, such as ensuring data security, maintaining the integrity of evidence, and navigating the ethical and legal implications of new forensic technologies. Issues related to privacy, consent, and potential bias in DNA databases are becoming increasingly complex as these systems expand. Furthermore, the legal admissibility of cutting-edge technologies like AI-driven DNA analysis and phenotypic prediction must be carefully evaluated to ensure the rigorous standards of forensic evidence in court are met.This review explores the opportunities and challenges associated with these emerging technologies, emphasizing the importance of responsible and ethical use. By examining advances in DNA extraction, spatial DNA analysis, and the integration of AI in forensic workflows, this manuscript provides forensic professionals with a roadmap for navigating the evolving landscape of forensic DNA analysis. The future of forensic DNA analysis lies in balancing technological innovation with the commitment to justice, ensuring that DNA evidence remains a reliable and indispensable tool in pursuing a more equitable legal system.

Use of Bibliometric Methods to Verify the Credentials of Expert Witnesses in Forensic Science and Legal Medicine and the Dilemma of Multiple-authorship

Expert testimony is an important part of criminal and civil litigation whenever scientific evidence needs to be explained and interpreted for the judge and jury. Those appearing in court as expert witnesses must possess the necessary qualifications, skill, training, and experience for the task in hand. Unlike a lay-witness, an expert witness is allowed to render an opinion based on their own specialized knowledge and research. In the adversarial system of justice, expert witnesses are hired by opposing sides in a case and this causes confusion when they disagree about the strengths and weaknesses of the scientific evidence presented. Choosing the best expert witness is often a difficult task and making a wrong decision has sometimes led to wrongful convictions and miscarriages of justice. Success in science is tightly linked to the quantity and quality of a person’s scholarly publications in academic journals in some particular area of research and scholarship. This article describes the use of a publicly available citation database to investigate the publication and citation records of British forensic practitioners with “legal and forensic medicine” (LFM) as their primary or secondary research category. How to attribute credit to individual names on multi-authored articles is a major problem in science and academia. Six different citation metrics, including authorship position on highly cited articles, were used to derive a composite citation score (c-score) for each highly cited scientist. Such bibliometric methods could prove useful in jurisprudence when reviewing the qualifications of people suggested to serve as expert witness in court cases.

Determining the Identity of Corpses Using Fingerprints: Results from Practice and Analysis of Process Used in the Republic of Serbia

In today’s world, when there is a constant fight against organized crime and terrorism, when we have cases of mass accidents (plane crashes, train crashes, buses, etc.), the constant need for precise and quick identification of persons is evident in these cases. When we have situations with a large number of dead in various conditions, as well as complete or only parts of the body being on the spot, there is a need to use scientific and forensic methods in order to find out the reliable identity of these people. Furthermore, there is a need, in some cases, to identify persons who committed suicide, were killed, or died a natural death (accidental death) and who do not have documents according to which their identity can be determined. The aim of this paper will, however, be to identify a group of persons who need to be identified, known as unidentified corpses. Method. Describe and discuss the way of determining identity based on dactyloscopic data, which provides accurate and unambiguous identification, using fingerprints. Results. The identity was determined in 1271 cases of unidentified corpses by dactyloscopic comparison of fingerprints with a database containing fingerprints of about 8,000,000 indisputably identified persons. It was confirmed in 1139 cases. Conclusion. The high degree of identification in our research, as much as 89.6%, makes this method rightly represented as a standard method for confirming a person’s identity.

Perspectives in Legal and Forensic Sciences: A New Open-Access Journal to Share Your Research on Legal and Forensic Sciences

Development and Validation of a Novel 29-plex STR Multiplex System for Legal and Forensic Science

Short tandem repeat (STR) analysis is the gold standard method in forensics for personal identification and paternity testing. Researchers have been working on developing novel multiplex systems containing more STRs for database construction and improvement of personal identification ability. This study's six-dye multiplex amplification system contained 29 autosomal loci, Y-indel, and Amelogenin. System optimization and performance measures were out according to the recommendations of the Scientific Working Group on DNA Analysis Methods, including PCR condition, sensitivity, mixture, inhibitor, species specificity, reproducibility, precision, stutter, concordance, and population study. The results showed that the complete profile was obtained with 125 pg of DNA input in a sensitivity study and a mixture ratio of 1:4. The full profile was observed with a hematin concentration ≤ 125 μmol/L, hemoglobin ≤ 200 μmol/L, EDTA ≤ 1.5 mmol/L, humic acid ≤ 1.5 μg/μL, indigo ≤ 12 mmol/L, and calcium ≤ 6.0 mmol/L. Meanwhile, the system also showed reasonable species specificity. Population genetic results showed the high performance of this panel with high informative and polymorphic loci, which possessed high estimates of the combined power of discrimination (1–7.16 × 10⁻³⁵) and the combined power of exclusion (1–1.98 × 10⁻¹²) in southern Han Chinese populations.

The Application of Forensic Imaging to Sex Estimation: Focus on Skull and Pelvic Structures

Forensic imaging is recognized as a vital tool in forensic practice mostly reflected by the wide use of post-mortem imaging in death investigations. With the surge of forensic imaging applications and research in recent years, many forensic subdisciplines have adopted this tool as a scientific investigation method, including forensic anthropology. Sex estimation is one of the key assignments in forensic anthropology along with age, ancestry and stature estimation. Traditionally, this assignment is done with non-metric macroscopic examination and metric analysis performed by forensic anthropologists. Today, forensic imaging serves as an auxiliary tool that adds to traditional methods and brings sex estimation to a dynamic era. The purpose of this article is to review forensic imaging methods in forensic anthropology sex estimation with a focus on skull and pelvic structures, aiming to provide insights into the best practices and prospective research directions. 

Principles in Identification of Human Remains through Forensic Odontology

Human identification is the core component of Forensic Odontology. The process of identification of unknown remains generally starts with the reconstruction of the skeletal biological profile, which provides a general description of the individual that is used to narrow down the candidates for the identity. Once one or more candidates are given for the identity, forensic odontologists conduct the comparison between the antemortem and postmortem records. The postmortem vs. the antemortem data comparison implies the evaluation of the consistencies and inconsistencies found in the data sets. This comparison is highly affected by the quality and completeness of the antemortem records, as well as the condition of the remains. The principles of the odontological comparison are based on the differences in the dental and maxillofacial structures due to human variation, development and pathology, and the alteration caused by dental treatment, which can be visually and radiographically observed. Restorative treatment, osseointegrated dental implants, fixed orthodontic and prosthetic appliances, along with dental and maxillofacial anatomy are the most informative features for the postmortem vs. antemortem comparison. The process of comparison consists of an objective identification of the consistencies and discrepancies. However, their interpretation and the final conclusion relies on the forensic odontologist knowledge and proficiency. Computer software packages such as WinID, DVI System International and UVIS can assist in the comparisons, connecting the postmortem and antemortem information and creating a ranking of possible matches. Moreover, deep machine learning models are being explored automate the comparison process. However, all comparison procedures still require the expert’s final assessment.

Postmortem Blood Metal Levels: Establishing Updated Reference Ranges Using ICP-OES

Postmortem testing for metals is crucial in forensic toxicology to determine whether metal exposure contributed to an individual’s death. However, current reference ranges for metal concentrations, primarily based on living individuals, fail to account for postmortem physiological changes. This study addresses this gap by analyzing thelevels of zinc and iron postmortem blood levels over various postmortem intervals (PMI) using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). Fifty samples were analyzed, revealing a significant increase in metal concentrations over time, with zinc levels rising from 181 μg/dL to 24,935 μg/dL and iron levels from 155 μg/dL to 11,421 μg/dL across a 40-month PMI. These changes are attributed to the redistribution of metals from tissues into the bloodstream and decomposition processes. The study proposes postmortem-specific reference ranges, emphasizing the need for forensic pathologists and toxicologists to consider PMI in their assessments to avoid misinterpretation and inaccurate cause-of-death determinations. This research underscores the necessity of updating reference ranges for postmortem analysis, ultimately improving the accuracy of forensic toxicology reports and contributing to more reliable determinations of cause of death.

Forensic Dental Age Estimation: Reliability Rating Compared to Clavicula

This study aimed to investigate the age determination in forensic expert opinions at the Institute of Forensic Medicine (Mainz) over the last ten years and to determine the reliability rate of wisdom teeth in comparison to the clavicle. A total of 112 expert opinions were prepared between 2011 and 2021, following the guidelines established by the Working Group for Forensic Age Diagnostics (AGFAD). Five indicators were studied: clavicle development coded according to Wittschieber et al. using computed tomography and wisdom tooth development 18, 28, 38 and 48 coded according to Demirjian’s staging method in a dental panoramic radiograph. Following an ordinary least square regression analysis performed separately for each of the five indicators, it was possible to investigate whether the addition of more than one of the indicators would lead to a more predictive value for the age determination. The combination of the clavicle and tooth 48 showed the best value. Adding tooth 38, which showed the second-best prediction in the bivariate analyses, led to an increase of the explained variance of 11% to a total of 58% explained variance (p < 0.001). The addition of further wisdom teeth did not show any relevant effect. For the clinical performance of dental age diagnostics, the teeth of the mandible, in combination with the clavicle, should be primarily used.

Improving Postmortem Dental Profiling: The Integration of Intraoral Scanners in Dental Autopsies

Forensic odontology plays a crucial role in human identification, particularly in cases where traditional identification methods face challenges such as severe trauma, decomposition, skeletonization, or carbonization. The evolution of digital dentistry has significantly advanced dental autopsies, particularly through the use of intraoral scanners (IOSs). These devices provide a non-invasive and efficient method for capturing detailed impressions of dentition and photographic images of teeth. The benefits of intraoral scanning in analyzing human remains in forensic odontology are endless. Digital impressions can be easily stored, shared, and transmitted electronically, eliminating the need for physical storage or transportation of dental models. This technology also enables remote postmortem dental profiling. By combining digital models with antemortem dental records, forensic odontologists can more efficiently identify matches and discrepancies, with the added benefit of future advancements in artificial intelligence(AI). Intraoral scanning should be considered a routine process in all dental autopsies to improve postmortem dental data collection and archive. Forensic odontologists should be equipped with a portable X-ray device, a digital sensor, and an IOS.

Determining the Identity of Corpses Using Fingerprints: Results from Practice and Analysis of Process Used in the Republic of Serbia

In today’s world, when there is a constant fight against organized crime and terrorism, when we have cases of mass accidents (plane crashes, train crashes, buses, etc.), the constant need for precise and quick identification of persons is evident in these cases. When we have situations with a large number of dead in various conditions, as well as complete or only parts of the body being on the spot, there is a need to use scientific and forensic methods in order to find out the reliable identity of these people. Furthermore, there is a need, in some cases, to identify persons who committed suicide, were killed, or died a natural death (accidental death) and who do not have documents according to which their identity can be determined. The aim of this paper will, however, be to identify a group of persons who need to be identified, known as unidentified corpses. Method. Describe and discuss the way of determining identity based on dactyloscopic data, which provides accurate and unambiguous identification, using fingerprints. Results. The identity was determined in 1271 cases of unidentified corpses by dactyloscopic comparison of fingerprints with a database containing fingerprints of about 8,000,000 indisputably identified persons. It was confirmed in 1139 cases. Conclusion. The high degree of identification in our research, as much as 89.6%, makes this method rightly represented as a standard method for confirming a person’s identity.utf-8

Use of Bibliometric Methods to Verify the Credentials of Expert Witnesses in Forensic Science and Legal Medicine and the Dilemma of Multiple-authorship

Expert testimony is an important part of criminal and civil litigation whenever scientific evidence needs to be explained and interpreted for the judge and jury. Those appearing in court as expert witnesses must possess the necessary qualifications, skill, training, and experience for the task in hand. Unlike a lay-witness, an expert witness is allowed to render an opinion based on their own specialized knowledge and research. In the adversarial system of justice, expert witnesses are hired by opposing sides in a case and this causes confusion when they disagree about the strengths and weaknesses of the scientific evidence presented. Choosing the best expert witness is often a difficult task and making a wrong decision has sometimes led to wrongful convictions and miscarriages of justice. Success in science is tightly linked to the quantity and quality of a person’s scholarly publications in academic journals in some particular area of research and scholarship. This article describes the use of a publicly available citation database to investigate the publication and citation records of British forensic practitioners with “legal and forensic medicine” (LFM) as their primary or secondary research category. How to attribute credit to individual names on multi-authored articles is a major problem in science and academia. Six different citation metrics, including authorship position on highly cited articles, were used to derive a composite citation score (c-score) for each highly cited scientist. Such bibliometric methods could prove useful in jurisprudence when reviewing the qualifications of people suggested to serve as expert witness in court cases.utf-8

Perspectives in Legal and Forensic Sciences: A New Open-Access Journal to Share Your Research on Legal and Forensic Sciences

Development and Validation of a Novel 29-plex STR Multiplex System for Legal and Forensic Science

Short tandem repeat (STR) analysis is the gold standard method in forensics for personal identification and paternity testing. Researchers have been working on developing novel multiplex systems containing more STRs for database construction and improvement of personal identification ability. This study's six-dye multiplex amplification system contained 29 autosomal loci, Y-indel, and Amelogenin. System optimization and performance measures were out according to the recommendations of the Scientific Working Group on DNA Analysis Methods, including PCR condition, sensitivity, mixture, inhibitor, species specificity, reproducibility, precision, stutter, concordance, and population study. The results showed that the complete profile was obtained with 125 pg of DNA input in a sensitivity study and a mixture ratio of 1:4. The full profile was observed with a hematin concentration ≤ 125 μmol/L, hemoglobin ≤ 200 μmol/L, EDTA ≤ 1.5 mmol/L, humic acid ≤ 1.5 μg/μL, indigo ≤ 12 mmol/L, and calcium ≤ 6.0 mmol/L. Meanwhile, the system also showed reasonable species specificity. Population genetic results showed the high performance of this panel with high informative and polymorphic loci, which possessed high estimates of the combined power of discrimination (1–7.16 × 10⁻³⁵) and the combined power of exclusion (1–1.98 × 10⁻¹²) in southern Han Chinese populations.utf-8

The Application of Forensic Imaging to Sex Estimation: Focus on Skull and Pelvic Structures

Forensic imaging is recognized as a vital tool in forensic practice mostly reflected by the wide use of post-mortem imaging in death investigations. With the surge of forensic imaging applications and research in recent years, many forensic subdisciplines have adopted this tool as a scientific investigation method, including forensic anthropology. Sex estimation is one of the key assignments in forensic anthropology along with age, ancestry and stature estimation. Traditionally, this assignment is done with non-metric macroscopic examination and metric analysis performed by forensic anthropologists. Today, forensic imaging serves as an auxiliary tool that adds to traditional methods and brings sex estimation to a dynamic era. The purpose of this article is to review forensic imaging methods in forensic anthropology sex estimation with a focus on skull and pelvic structures, aiming to provide insights into the best practices and prospective research directions. utf-8

Emerging Technologies in Forensic DNA Analysis

Forensic DNA analysis has fundamentally transformed criminal investigations, providing an unprecedented level of accuracy in identifying suspects, exonerating the innocent, and solving cold cases. This manuscript reviews the emerging technologies that are reshaping the field of forensic DNA analysis, including next-generation sequencing (NGS), rapid DNA analysis, AI-driven forensic workflows, 3D genomics, and mobile DNA platforms. These innovations enhance the speed, precision, and scope of DNA analysis, allowing forensic scientists to process evidence more efficiently, analyze more complex samples, and conduct real-time field-based investigations. While these advancements hold great promise, they also introduce significant challenges, such as ensuring data security, maintaining the integrity of evidence, and navigating the ethical and legal implications of new forensic technologies. Issues related to privacy, consent, and potential bias in DNA databases are becoming increasingly complex as these systems expand. Furthermore, the legal admissibility of cutting-edge technologies like AI-driven DNA analysis and phenotypic prediction must be carefully evaluated to ensure the rigorous standards of forensic evidence in court are met.This review explores the opportunities and challenges associated with these emerging technologies, emphasizing the importance of responsible and ethical use. By examining advances in DNA extraction, spatial DNA analysis, and the integration of AI in forensic workflows, this manuscript provides forensic professionals with a roadmap for navigating the evolving landscape of forensic DNA analysis. The future of forensic DNA analysis lies in balancing technological innovation with the commitment to justice, ensuring that DNA evidence remains a reliable and indispensable tool in pursuing a more equitable legal system.utf-8

Principles in Identification of Human Remains through Forensic Odontology

Human identification is the core component of Forensic Odontology. The process of identification of unknown remains generally starts with the reconstruction of the skeletal biological profile, which provides a general description of the individual that is used to narrow down the candidates for the identity. Once one or more candidates are given for the identity, forensic odontologists conduct the comparison between the antemortem and postmortem records. The postmortem vs. the antemortem data comparison implies the evaluation of the consistencies and inconsistencies found in the data sets. This comparison is highly affected by the quality and completeness of the antemortem records, as well as the condition of the remains. The principles of the odontological comparison are based on the differences in the dental and maxillofacial structures due to human variation, development and pathology, and the alteration caused by dental treatment, which can be visually and radiographically observed. Restorative treatment, osseointegrated dental implants, fixed orthodontic and prosthetic appliances, along with dental and maxillofacial anatomy are the most informative features for the postmortem vs. antemortem comparison. The process of comparison consists of an objective identification of the consistencies and discrepancies. However, their interpretation and the final conclusion relies on the forensic odontologist knowledge and proficiency. Computer software packages such as WinID, DVI System International and UVIS can assist in the comparisons, connecting the postmortem and antemortem information and creating a ranking of possible matches. Moreover, deep machine learning models are being explored automate the comparison process. However, all comparison procedures still require the expert’s final assessment.utf-8

Postmortem Blood Metal Levels: Establishing Updated Reference Ranges Using ICP-OES

Postmortem testing for metals is crucial in forensic toxicology to determine whether metal exposure contributed to an individual’s death. However, current reference ranges for metal concentrations, primarily based on living individuals, fail to account for postmortem physiological changes. This study addresses this gap by analyzing thelevels of zinc and iron postmortem blood levels over various postmortem intervals (PMI) using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). Fifty samples were analyzed, revealing a significant increase in metal concentrations over time, with zinc levels rising from 181 μg/dL to 24,935 μg/dL and iron levels from 155 μg/dL to 11,421 μg/dL across a 40-month PMI. These changes are attributed to the redistribution of metals from tissues into the bloodstream and decomposition processes. The study proposes postmortem-specific reference ranges, emphasizing the need for forensic pathologists and toxicologists to consider PMI in their assessments to avoid misinterpretation and inaccurate cause-of-death determinations. This research underscores the necessity of updating reference ranges for postmortem analysis, ultimately improving the accuracy of forensic toxicology reports and contributing to more reliable determinations of cause of death.utf-8

Forensic Dental Age Estimation: Reliability Rating Compared to Clavicula

This study aimed to investigate the age determination in forensic expert opinions at the Institute of Forensic Medicine (Mainz) over the last ten years and to determine the reliability rate of wisdom teeth in comparison to the clavicle. A total of 112 expert opinions were prepared between 2011 and 2021, following the guidelines established by the Working Group for Forensic Age Diagnostics (AGFAD). Five indicators were studied: clavicle development coded according to Wittschieber et al. using computed tomography and wisdom tooth development 18, 28, 38 and 48 coded according to Demirjian’s staging method in a dental panoramic radiograph. Following an ordinary least square regression analysis performed separately for each of the five indicators, it was possible to investigate whether the addition of more than one of the indicators would lead to a more predictive value for the age determination. The combination of the clavicle and tooth 48 showed the best value. Adding tooth 38, which showed the second-best prediction in the bivariate analyses, led to an increase of the explained variance of 11% to a total of 58% explained variance (p < 0.001). The addition of further wisdom teeth did not show any relevant effect. For the clinical performance of dental age diagnostics, the teeth of the mandible, in combination with the clavicle, should be primarily used.utf-8

Improving Postmortem Dental Profiling: The Integration of Intraoral Scanners in Dental Autopsies

Forensic odontology plays a crucial role in human identification, particularly in cases where traditional identification methods face challenges such as severe trauma, decomposition, skeletonization, or carbonization. The evolution of digital dentistry has significantly advanced dental autopsies, particularly through the use of intraoral scanners (IOSs). These devices provide a non-invasive and efficient method for capturing detailed impressions of dentition and photographic images of teeth. The benefits of intraoral scanning in analyzing human remains in forensic odontology are endless. Digital impressions can be easily stored, shared, and transmitted electronically, eliminating the need for physical storage or transportation of dental models. This technology also enables remote postmortem dental profiling. By combining digital models with antemortem dental records, forensic odontologists can more efficiently identify matches and discrepancies, with the added benefit of future advancements in artificial intelligence(AI). Intraoral scanning should be considered a routine process in all dental autopsies to improve postmortem dental data collection and archive. Forensic odontologists should be equipped with a portable X-ray device, a digital sensor, and an IOS.utf-8