Artiles
Review
28 March 2024Digital Twins Enabling Intelligent Manufacturing: From Methodology to Application
Digital twin technology develops virtual models of objects digitally, simulating their real-world behavior based on data. It aims to reduce product development cycles and costs through feedback between the virtual and real worlds, data fusion analysis, and iterative decision-making optimization. Traditional manufacturing processes often face challenges such as poor real-time monitoring and interaction during machining, difficulties in diagnosing equipment failures, and significant errors in machining. Digital twin technology offers a powerful solution to these issues. Initially, a comprehensive review of the research literature was conducted to assess the current research scope and trends. This was followed by an explanation of the basic concepts of digital twins and the technical pathway for integrating digital twins into intelligent manufacturing including outlining the essential technologies for creating a system of interaction between the virtual and real worlds, enabling multimodel fusion, data sensing, algorithm-based prediction, and intelligent decision-making. Moreover, the application of digital twins in intelligent manufacturing throughout the product life cycle was detailed, covering product design, manufacturing, and service stages. Specifically, in the manufacturing phase, a model based on heat conduction theory and visualization was used to construct a time-varying error model for the motion axis, leading to experiments predicting the time-varying error in the hole spacing of a workpiece. These experiments achieved a minimum prediction error of only 0.2 μm compared to the actual error. By compensating for time-varying errors in real time, the variability in the hole spacing error decreased by 69.19%. This paper concludes by summarizing the current state of digital twins in intelligent manufacturing and projecting future trends in key technologies, application areas, and data use, providing a basis for further research.
Review
27 March 2024Research Progress on Electrolyte Additives for Sodium Ion Batteries
In view of the gradual depletion of lithium resources, sodium-ion batteries (SIBs) have emerged as a viable alternative to lithium-ion batteries (LIBs). This is primarily attributed to their comparable operational principles and abundant reserves of sodium resources. As an essential component of the secondary battery, the electrolyte is of paramount importance in the functioning of SIBs, and the electrode-electrolyte interface constructed by it affects the battery performance. Adding electrolyte additives in LIBs is a low-cost and efficient method that can enhance the performance of the electrolyte and the interface between the electrode and electrolyte. This method is also applicable to SIBs. Therefore, in this study, we provide a comprehensive overview of various electrolyte additives, including but not limited to carbonate additives, sulfur-containing additives, silicon-containing additives, phosphorus-containing additives and inorganic additives. We extensively analyze the impact of these additives on the electrode-electrolyte interface and the electrochemical performance of SIBs. The purpose of this review is to comprehensively evaluate the current status of electrolyte additives in SIBs, which serves as both a basic overview of the existing situation and a practical guide for selecting suitable additives for practical applications of SIBs.
Article
25 March 2024Proteomic Analysis of Pleurotus ostreatus Grown on Glucose and Xylose Mixtures in Submerged Fermentation Provides Insights into Differentiated Mycelial Composition
Pleurotus ostreatus, an edible white-rot fungus of great commercial and nutritional value, grows by metabolizing mainly glucose and xylose, the two major sugars in lignocellulosic biomass. In this study, a comparative proteomic analysis of P. ostreatus grown in submerged fermentation on a medium with glucose, xylose and mixtures of them as carbon sources was conducted. In the same conditions, the metabolic response of the fungus was evaluated in the production of the main nutritional components of the fungus such as proteins, lipids, and intracellular and extracellular polysaccharides. The proteomic analysis revealed that glucose and xylose upregulate different clusters of proteins. Glucose mainly up-regulates macromolecule metabolic processes, translation and glycolysis whereas xylose up-regulates, small molecule metabolic processes and tricarboxylic acid cycle (TCA). The mixtures show mostly similarities with the proteome response to glucose, although there are differential responses depending on xylose concentration. The carbon source type found to affect the basic macromolecule metabolic processes, with amino acids biosynthesis to differentiate mostly. An analysis of the upregulated proteins through the STRING database revealed that xylose upregulates mostly proteins related to amino acid biosynthesis. Leucine, Valine and Isoleucine biosynthesis pathways were found to be the most triggered pathway. All the branched-chain amino acids (BCAAs)-related enzymes intensities were gradually increased when xylose concentration was increased in the growth medium. BCAAs play an important role in the human diet so the enhancement of BCAAs biosynthesis pathway for P. ostreatus could convert it to a very remarkable protein substitute in human diet. These findings provide new insights into the proteomic and metabolic response of the fungus to the major sugars of lignocellulosic biomass, which are not well understood until now.
Article
25 March 2024Discussion on the Marine Protected Area on the High Seas: From the Perspective of Obligations Erga Omnes Partes
The BBNJ Agreement promotes the conservation and sustainable use of high seas marine biodiversity through the establishment of high seas protected areas. The high seas biodiversity protected by the Agreement has the nature of “obligations erga omnes partes” on an ex officio basis, but in judicial practice it is subject to a finding by the International Court of Justice that the adoption of treaty-based institutional arrangements is in the “collective interest” and that it is in the “collective interest” to adopt such arrangements. The BBNJ Agreement is currently not a “collective interest” agreement in terms of the management of the BBNJ Agreement. At present, the hybrid management model adopted in the BBNJ Agreement does not reflect the collective interest in substance, and cannot resolve the conflict between the establishment of protected areas on the high seas and other area-based management tools, so it is necessary to further harmonize the relationship between the Conference of Parties to the BBNJ and the IFB, and to strengthen the mandate of the COP.
Article
22 March 2024Modulation of the MEP Pathway for Overproduction of 13-R-manoyl Oxide in Cyanobacteria
The cyanobacterium Synechocystis sp. PCC 6803 has gained scientific interest for its potential to use solar energy and atmospheric CO2 for the production of high-value chemicals like pharmaceuticals, flavors, and fragrances. Forskolin is a diterpenoid found in the root cork of the plant Plectranthus barbatus and its biosynthetic pathway is initiated by two terpene synthases that convert geranylgeranyl diphosphate (GGDP) into the precursor 13-R-manoyl oxide (13-R-MO). Using the cyanobacterium Synechocystis sp. PCC 6803 as host, we expressed the two terpene synthases resulting in the synthesis of 0.83 mg/L 13-R-MO. Three different geranylgeranyl diphosphate synthases (GGDPSs) were selected for screening; a prokaryotic (Synechococcus sp. JA-3-3Ab (Sj)), a yeast (Saccharomyces cerevisiae (Sc)), and a plant (P. barbatus (Pb)) derived GGDPS. Strains containing the prokaryotic Sj- or the yeast ScGGDPS consistently yielded more 13-R-MO than the base strain. By overexpression of 1-Deoxy-D-xylulose-5-phosphate synthase (DXS) positioned at the entry of the 2-C-methyl-d-erythritol 4-phosphate pathway (MEP) together with the prokaryotic SjGGDPS, the 13-R-MO titer was increased 11-fold to reach 9.7 mg/L by boosting the synthesis of GGDP, the direct substrate for the diterpenoid synthases. We further show that application of a n-dodecane overlay to remove 13-R-MO from the culture medium provided a 2–3 fold increase of the 13-R-MO in a separate cultivation system.
Article
22 March 2024Community Sport, Australian Sport Policy and Advocacy: A Qualitative Study of Stakeholder Perspectives
This article explored aspects of the community sport policy process in rural New South Wales, Australia, focusing on the views of community sport club (CSC) officials relating to policy matters. Community sport represents a complicated policy arena, and rural communities face a level of disparity compared with better-resourced urban CSCs, particularly concerning policy implementation and advocacy issues. Officials at CSCs from ten different sports (n = 10) in a rural setting participated in semi-structured interviews to pinpoint themes common in the community sport policy process. Further, the research identified aspects of the connections that impact CSCs, including those with government and National Sporting Organisations (NSOs). To highlight the beliefs and attitudes of the CSC officials, the interviews had two key thematic foci—implementation and advocacy—and the findings highlighted sub-themes relating to the fundamental interests of CSCs. Overall, the research accentuated the hierarchical nature—a power imbalance—of sport policy processes, the potential for CSCs to have a bottom-up role in policy creation, and the consideration of a policy analysis and evaluation structure such as the Advocacy Coalition Framework. Finally, the outcome points to enthusiasm for strengthening community sport by giving CSCs a voice through localized advocacy.
Article
20 March 2024Development and Validation of a Novel 29-plex STR Multiplex System for Legal and Forensic Science
Short tandem repeat (STR) analysis is the gold standard method in forensics for personal identification and paternity testing. Researchers have been working on developing novel multiplex systems containing more STRs for database construction and improvement of personal identification ability. This study's six-dye multiplex amplification system contained 29 autosomal loci, Y-indel, and Amelogenin. System optimization and performance measures were out according to the recommendations of the Scientific Working Group on DNA Analysis Methods, including PCR condition, sensitivity, mixture, inhibitor, species specificity, reproducibility, precision, stutter, concordance, and population study. The results showed that the complete profile was obtained with 125 pg of DNA input in a sensitivity study and a mixture ratio of 1:4. The full profile was observed with a hematin concentration ≤ 125 μmol/L, hemoglobin ≤ 200 μmol/L, EDTA ≤ 1.5 mmol/L, humic acid ≤ 1.5 μg/μL, indigo ≤ 12 mmol/L, and calcium ≤ 6.0 mmol/L. Meanwhile, the system also showed reasonable species specificity. Population genetic results showed the high performance of this panel with high informative and polymorphic loci, which possessed high estimates of the combined power of discrimination (1–7.16 × 10−35) and the combined power of exclusion (1–1.98 × 10−12) in southern Han Chinese populations.
Article
19 March 2024Designing a Quadcopter for Fire and Temperature Detection with an Infrared Camera and PIR Sensor
In agriculture, medicine, and engineering, sudden fire outbreaks are prevalent. During such events, the ensuing fire spread is extensive and unpredictable, necessitating crucial data for effective response and control. To address this need, the current initiative focuses on utilizing an Unmanned Aerial Vehicle (UAV) with an Infrared (IR) sensor. This sensor detects and analyses temperature variations, accompanied by additional camera footage capturing thermal images to pinpoint the locations of the incidents precisely. The UAV’s programming is executed using Arduino-Nano and mission planner software, interfacing with the Pixhawk flight controller operating in a guided mode for autonomous navigation. The UAV configuration includes a radio module interfacing with Arduino-Nano, a flight controller, and remote-control functionality. The flight duration is approximately 10–15 min, contingent upon flight dynamics and environmental temperature. Throughout its airborne operation, the UAV transmits live telemetry and log feeds to the connected computer, displaying critical parameters such as altitude, temperature, battery status, vertical speed, and distance from the operator. The Pixhawk flight controller is specifically programmed to govern the UAV’s behavior, issuing warnings to the pilot in case of low voltage, prompting a timely landing to avert potential crashes. In case of in-flight instability or a crash, the mission planner can trace the UAV’s location, facilitating efficient recovery and minimizing costs and component availability losses. This integrated approach enhances situational awareness and mitigation strategies, offering a comprehensive solution for managing fire incidents in diverse fields.
Article
13 March 2024The Potential of Salinity Gradient Energy Using Reverse Electrodialysis to Generate Electricity for Seawater Desalination Plants, an Example from Western Australia
Seawater desalination plays a vital role in addressing the increasing global demand for freshwater. However, the energy-intensive nature of desalination processes and the generation of brine by-products pose environmental challenges. In Western Australia (WA), approximately 48% of freshwater is supplied by two seawater desalination plants employing the energy-intensive seawater reverse osmosis (SWRO) method. These plants are powered by a combination of renewable and conventional energy sources. Typically, the most efficient approach for desalination plants involves a blend of renewable energy sources. Salinity gradient energy (SGE) harnessed through the reverse electrodialysis (RED) system, which derives energy from mixing waters with varying salinities, has emerged as a potential solution. RED utilizes ion-exchange membranes to convert the chemical potential difference between two solutions into electric power. The net specific energy of SGE, calculated based on the Gibbs free energy associated with mixing seawater and wastewater, is estimated at approximately 0.14 kWh per cubic metre of brine for SWRO desalination plants. The combined SGE potential of WA’s two desalination facilities theoretically amounts to approximately 87.4 MWh of energy. However, due to the inherent limitations of the RED system’s current energy efficiency, only about 2.5% of the desalination plant’s energy requirements can be met through this technique. This paper addresses a significant gap in the literature by analyzing the technical and economic constraints of utilizing salinity gradient energy (SGE) through the reverse electrodialysis (RED) system for seawater desalination plants. This marks the first examination of its kind, shedding light on both the technical feasibility and economic challenges of SGE-RED application in this context. The scientific contribution lies in its innovative approach, integrating technical and economic perspectives to provide an understanding of SGE-RED technology’s potential drawbacks and opportunities. By identifying and tackling these challenges, this paper aims to pave the way for optimizing SGE-RED systems for practical implementation in seawater desalination plants.
Article
08 March 2024Green Synthesis of Gold Nanoparticles for Catalytic Reduction of 4-Nitrophenol and Methylene Blue for Sustainable Development
Unique structural features and wide applications of gold nanoparticles (GNPs) are inspiring researchers to develop biocompatible, reliable and cost-effective methods for their synthesis. Herein, a clean, eco-friendly and non-toxic method to obtain GNPs was developed by reducing and capping the liquid extract of stem of Lilium longiflorum and highlights the catalytic reduction of 4-nitrophenol (4-NP) and methylene blue (MB). The formation of GNPs was confirmed through the absorption peak at 535 nm in the UV-Vis spectra. TEM and HRTEM analyses reveal GNPs spherical morphology with an average size of 4.97 nm. SEM and EDX analyses further elucidate the spherical nature of GNPs and elemental composition. FTIR spectroscopy analysis demonstrates that the GNPs were coated with organic compounds, which prevent the nanoparticle from aggregation. GNPs exhibit remarkable efficiency in reducing 4-NP and MB. The catalytic efficacy of the synthesized GNPs was demonstrated through the enhanced reduction rates of 4-NP and MB, with rate constants of 1.50 min−1 and 1.29 min−1, respectively. This study develops a novel and eco-friendly technique for the synthesis of gold nanoparticles and opens possibilities for the green synthesis of other metal nanoparticles. The confirmed catalytic activity holds promise for a range of industrial applications and environmental sustainability.
