The dysregulation of microbial communities poses severe threats to host health and ecological stability, yet traditional microbiome modulation strategies lack specificity and often exacerbate dysbiosis. The CRISPR-Cas system offers unprecedented potential for targeted microbiome regulation due to its sequence-specific nucleic acid recognition and cleavage capabilities, but its translation is hindered by inefficient and non-specific delivery. As natural bacterial predators with inherent host specificity, bacteriophages have emerged as ideal carriers to address this delivery bottleneck. The development of CRISPR-armed phages combines the targeted delivery of phages and the precision editing advantages of CRISPR-Cas systems. This review systematically elaborates on the design and mechanism of CRISPR-armed phages for microbiome engineering. CRISPR-Cas systems were classified on the basis of their structural and functional characteristics, as well as their regulatory effects, in detail after phage delivery. The engineering strategies of integrative and non-integrative phage vectors were then discussed, followed by their applications in ecological regulation and genetic regulation of the microbiome. The current limitations were finally analyzed, such as narrow phage host range, bacterial phage resistance, and low editing efficiency. This review provides a comprehensive theoretical framework to promote the development of CRISPR-armed phages, aiming to advance precision microbiome engineering for human health.
Chloride intracellular ion channels (CLICs) represent a relatively underexplored class of chloride channels and are included in a research initiative that focuses on druggable genes that have not been well studied yet. As a unique family, CLICs exist in membrane and soluble forms and play a role in regulating chloride flux and modulating various aspects of cellular biology. To date, six mammalian CLICs have been cloned and characterized at molecular and physiological levels. The respiratory system, responsible for gas exchange between the atmosphere and the human body, has recently been shown to express CLICs with functional relevance in lung pathophysiology, including lung carcinoma, inflammation, and endothelial dysfunction. Notably, the expression patterns of CLIC isoforms in lung cell types are distinct. Among them, CLIC1, CLIC3, and CLIC4 have been investigated more extensively, particularly in the context of lung cancer, inflammatory diseases, and pulmonary arterial hypertension. A deeper understanding of the role of CLICs in regulating lung cellular function may pave the way for developing novel therapeutic strategies to treat pulmonary disorders. In this review, we summarize the expression and functional roles of CLICs in lung pathophysiology, with particular emphasis on CLIC1, CLIC3, and CLIC4.
Soft robotics has emerged as a promising direction for enabling safe, adaptive, and energy-efficient interactions with unstructured environments due to its inherent compliance. Recently, Deep Reinforcement Learning (DRL) has become a powerful tool for autonomous behavior generation in soft robots, surpassing limitations of classical model-based control. However, despite rapid growth of publications in this domain, there is still a lack of systematic comparative surveys that clarify how different DRL approaches have been used for soft mobile robots, what types of tasks they address, and what performance evaluation criteria have been used. In this article, we review and classify existing works in DRL-enabled soft robotics, focusing particularly on soft mobile systems, and present a structured synthesis of contributions, algorithms, training strategies, and real-world applications. Unlike previous reviews that discuss soft robotics or DRL separately, this paper explicitly provides cross-comparison across DRL paradigms and soft robot tasks, enabling researchers to identify suitable DRL approaches for different soft mobile robotic behaviors. Finally, major challenges and promising future directions are proposed to advance this interdisciplinary research area.
Steel is an essential component used to build marine vessels due to its endurance of the sea’s harsh conditions, including corrosion and dynamic stresses, therefore, different grades of mild steel are used in shipbuilding. It provides the strength, ductility, and weldability necessary for structural integrity, consisting of carbon, manganese, etc., as alloying elements. In this research, different quenching media were employed to assess variations in mechanical properties. This process ultimately triggered alterations in the microstructure of the steel. Two types of media, such as vegetable oil (Canola) and Polyvinylpyrrolidone polymer (PVP), were studied in comparison with simple heat-treated steel. Mechanical characterization comprised of tensile testing, hardness and impact testing to evaluate major changes in strength and ductility. Furthermore, a microscope was used to interpret the microstructure. To guarantee consistency in testing, samples were prepared in accordance with ASTM guidelines. The yield strength of as-received steel was increased from 298 MPa to 358 MPa and 370 MPa because of rapid cooling action in PVP and oil, respectively. A significant increase in Ultimate tensile strength was achieved due to the variety of quenching media; however, ductility was seriously compromised because of the excessive hardness of the material. Impact energy analysis revealed a notable reduction, which is linked with degradation in toughness.
The efficiency of lignocellulosic biorefineries is limited because of the high recalcitrance and low reactivity of lignin. The reactivity of lignin can be enhanced through various chemical and biochemical approaches. Demethylation is one of the methods that improve the availability of phenolic hydroxyl groups in lignin, thereby enhancing its reactivity and application in sustainable adhesives. The goal of this study is to integrate microbial and chemical approaches to aid in the demethylation of lignin. Towards that end, lignin was first extracted and purified from the rice straw biorefinery solid residue obtained post ethanol fermentation. This rice straw lignin was then subjected to chemical and microbial demethylation. For microbial demethylation under alkaline conditions, Pseudomonas putida and Pseudomonas fluorescens were employed, while demethylation under neutral conditions was conducted using Trametes versicolor. Integrated treatment using Pseudomonas putida followed by hydrogen iodide yielded an increase in the phenolic hydroxyl content by approximately 39–43%. Demethylation using chemical methods and biological methods alone provided approximately 18–27% increases in phenolic hydroxyl content, respectively. Furthermore, to assess the physical and chemical properties of demethylated lignin, FT-IR, TGA, and morphological analytical tools were employed.
