Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive fibrotic lung disease with a poor prognosis. Previous research has revealed that the gut microbiota is associated with human health and immunity, and it interacts with the lung through the “gut-lung axis”. This study explores the potential relationship between Gut Microbiome Metabolites and Idiopathic pulmonary fibrosis via Network Pharmacology Study. The metabolites from gut microbiota were retrieved from the gutMGene database, and gene targets for these metabolites were obtained from previous studies. Gene targets of IPF were obtained from public databases (DisGeNET, OMIM). Subsequently, following the identification of shared targets, IL6 was determined as the core target through protein-protein interaction analysis. Then, a microbiota-metabolite-target-signaling pathway network (MMTS) was constructed using Cytoscape 3.10, and targets with low expression in the lungs and intestines were deleted. The MMTS network revealed that three short-chain fatty acids—acetate, butyrate, propionate, and a flavonoid compound called equol—are IL6-related metabolites. Then, we performed a molecular docking test (MDT) using CB-Dock2 to validate the affinity between core targets and metabolites. MDT confirmed that equol produced by the conversion of isoflavones from Lactobacillus paracasei JS1 was more stable in binding to IL6 than the other three short-chain fatty acids, thereby affecting multiple signaling pathways and influencing the progression of IPF. Finally, we validated this hypothesis through in vitro cell culture experiments, further confirming the effect of Equol.
Asthma affects millions worldwide and involves complex genetic, immunological, and environmental factors. The nasal microbiome is increasingly recognized for its role in asthma development, but inconsistent results and small sample sizes have limited a clear understanding. We aimed to clarify the nasal microbiome’s role in asthma using large datasets and meta-transcriptomic analysis. RNA-seq data was analyzed from two large public studies: GALA II (694 children of Puerto Rican heritage; 441 asthmatics, 253 controls) and CAAPA (562 individuals of African ancestry; 265 asthmatics, 297 controls). After quality control and host read removal, microbial reads were annotated using Kraken2. α and β diversity analyses compared microbial diversity between asthmatic and control groups. Differential abundance analysis was conducted separately, controlling for age and sex, with results combined via meta-analysis. We found that asthmatic patients exhibited significantly higher α diversity indices (Shannon, Berger-Parker, Inverse Simpson, Fisher’s) in nasal microbiota compared to controls in GALA II, with similar trends in CAAPA. β diversity analysis showed significant differences in microbial composition in GALA II data. Differential abundance analysis identified 20 species in GALA II and 9 species in CAAPA significantly associated with asthma. Meta-analysis revealed 11 species significantly associated with asthma, including Mycobacterium_tuberculosis. Our study demonstrates increased nasal microbiome α diversity in asthmatic patients and identifies specific microbial species associated with asthma risk. These findings enhance understanding of asthma pathogenesis from the nasal microbiome perspective and may inform future research and therapeutic strategies.
China’s ongoing supply-side structural reforms and the government’s strategic focus on rural revitalization catalyze a significant transformation in rural landscapes. The steady rise in rural residents’ incomes and assets, along with gradual improvements in household quality of life, is unlocking significant potential for growth and upgrades in the rural consumer market.This evolution necessitates a more diversified and sophisticated approach to financial services that is more inclusive and widespread. Despite the challenges, such as an aging population and imperfect credit systems that have limited the quality and precision of rural financial services, the analysis of household income and asset structures is vital for understanding consumption patterns and enhancing the economic vitality of rural households. Our study, based on the 2019 China Household Finance Survey data of 11,386 households and employing the Heckman two-stage model, finds that the proportions of income components and financial assets, moving in opposite directions, significantly influence the amount of consumer credit demanded, while other asset types, such as real estate, show no significant effect. The examination of income and asset diversity through the Herfindahl-Hirschman Index (HHI) demonstrates varying impacts of income and asset structures on credit demand, depending on the concentration levels of income and assets. Additionally, our analysis of heterogeneity based on the age of the household head and geographic region highlights the diverse influences on the amount of consumer credit demand, underscoring the importance of tailoring financial products and policies to meet the specific needs of rural households. These findings shed light on these dynamics and inform financial service innovations that align with the needs of rural consumers, thereby supporting the broader objectives of rural economic development and the achievement of China’s rural revitalization strategies.
In recent decades, countries have experienced a widespread increase in population that has resulted in unorganized urban sprawl, problems with land use change and related threats to natural resources. On the other hand, this led to converting agricultural areas into urban spaces. This work aims to analyze land use changes in the metropolitan area of Rome, which has experienced significant urbanization in rural areas.In this context, the agricultural sector’s contribution to the economic growth of peri-urban areas in the metropolitan area of Rome was verified. After showing the extent of agricultural land consumption, an economic evaluation of land use was provided through the analysis of the evolution of urban and peri-urban agriculture in Rome. The analysis was performed using the Italian FADN data for 2008–2020 and a set of structural and economic–financial indicators. Furthermore, the study analyzes the relationship between farm performance and agricultural resources, farmer demographics, and farm size. The analysis reveals that farms are mainly specialized in arable land and herbivores and are conducted prevalently by men with a high school education level and aged between 40 and 65 years. The economic results also show a good performance; however, they are yearly differentiated. Only a few farms resort to other gainful activities to increase the income produced. Therefore, a greater diversification of agricultural activities is desirable. The research provides interesting insights to stakeholders on the public support that needs to be designed and implemented to favor the survival of farms in rural areas.
The growing demand for sustainable materials in the automotive industry has prompted research into natural fiber-reinforced composites. To reduce carbon footprints and enhance product sustainability, the sectors increasingly focus on renewable and biodegradable materials. Composites made from natural fibers, such as coir and hemp, offer a promising solution for creating lightweight, high-performance components with a reduced environmental impact.In this study, an experimental investigation was conducted to examine the impact of single and hybrid and treated and untreated fibers, on the properties of epoxy-based composites. Untreated hemp fiber with treated Coir fiber was used for the research. The composites were fabricated through the open mould hand lay-up technique. Samples were prepared by randomly dispersing the fibers in the epoxy matrix before pouring them into the respective moulds prepared according to ASTM standards. Tensile, impact, and hardness tests were conducted on the cured samples to determine their mechanical properties, while a scanning electron microscope was used to evaluate the fractured surface. Water absorption tendencies were also determined. The results showed that the sample denoted as 5CF wt.% had the best property combination with tensile strength (32.4 MPa), tensile modulus (11.9 GPa), flexural strength (167.0 MPa), and impact strength (46.8 kJ/mm2). It was discovered that hemp fiber-based composites were not enhanced properly due to lack of fiber surface modifications. Though optimum results were obtained from treated coir fiber-based single/distinct composite, untreated hemp fiber was discovered to aid some flexural modulus and hardness properties in the hybrid composite based on the best results obtained in its distinct-based composite. Therefore, untreated hemp fiber can be used in hybrid form with treated coir fiber where one of the fibers is scarce or when fiber surface medication is difficult to achieve. Thus, the results showed that 5CH-based composites are the most suitable composition for automotive components development where high-mechanical properties are essential.
